FastQCFastQC Report
Mon 27 Feb 2023
SRR3126447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890311
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489751.6944543338069848No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37750.13060878223831277No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC33610.11628506413323687TruSeq Adapter, Index 10 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT32740.1132750074299963TruSeq Adapter, Index 10 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG32350.11192567166647466RNA PCR Primer, Index 10 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT30270.1047292142610259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168050.086.563441
CGTAGGG8550.074.208312
ATAGGGC18100.072.186943
GTAGGGA18950.071.9252243
ACGGGAT8550.071.46114
GTAGGGT11150.071.236773
AGTAGGG45750.070.78042
TAGGGTA9700.070.741384
TAGGGCA14800.070.499124
ATAGGGA23650.068.760243
TAGCGGG15800.068.1182252
ACGGGTA2900.068.0681154
GTACGGG6400.067.5604862
TAGGGAT15650.067.270724
TAGAGGG52500.066.8722842
AGGGATG37500.066.801835
TAAGAGG19700.066.608721
AATAGGG40700.066.2830352
TATAGGG24600.066.103722
AAGAGGG94250.065.8229752
Produced by FastQC (version 0.11.5)