FastQCFastQC Report
Mon 27 Feb 2023
SRR3126446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399220
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238770.995198439492835No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53830.22436458515684263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83550.085.068661
TAGACGG3150.074.71341
AGTAGGG42150.071.961882
ACGGGAT5950.071.877544
ATAGGGC14800.071.130373
AGGGACT38350.070.954845
TAGGGTA8300.070.77844
GTAGGGA17600.070.762313
TAGCGGG14350.070.457452
AGGGATG30750.069.998565
ATAGCGG6950.069.080341
ATAAGGG32000.069.069812
AATAGGG33000.068.829342
ACTAGGG12450.068.3674852
GAATAGG12600.067.98921
TAGGGCA13150.067.90424
ATGCGGG12750.067.865342
AAGGGAC23450.067.539194
TAGGGTC6850.067.2363744
CGTAGGG5250.067.180372
Produced by FastQC (version 0.11.5)