FastQCFastQC Report
Mon 27 Feb 2023
SRR3126445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527287
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240910.9532356238132037No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53960.21350958557536204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81800.084.867941
TAGGGTA8500.072.977244
ATAGGGC15850.072.3424153
AGGGATG31800.072.1149145
ATAGGGA18450.071.062463
AGTAGGG42400.070.6002042
TAGCGGG13000.070.489372
TAGGGCA12800.069.755114
GTACGGG6200.069.731432
AGTACGG3600.069.244291
ATAGAGG15950.068.707771
GAATAGG15100.068.214671
GTAGGGA18100.068.0228963
TAGGGAC12450.067.941574
GTAGGGC13000.066.8745353
AATAGGG36350.066.837242
AGGGACT40600.066.554015
ACTAGGG12600.066.386822
ATAAGGG35400.066.3741762
GGTAAGG16500.066.132461
Produced by FastQC (version 0.11.5)