FastQCFastQC Report
Mon 27 Feb 2023
SRR3126444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821225
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305231.0819059096669No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60840.21565100266728107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100050.085.889831
ATAGGGC18050.074.468113
GAATAGG17500.071.761831
AGTAGGG50000.071.437312
TAGGGTA11300.070.289384
AGGGACT46250.070.2178965
TAGGGCA15300.070.0365754
AGGGATG36400.069.722655
TAGAGGG50800.069.572182
TAGCGGG16900.069.5242
TAGGGAC14100.069.330724
AGATAGG15100.068.839171
AATAGGG40600.068.297922
ATAGGGA20750.067.949253
ATAGGGT11500.067.840923
GTAGGGC15250.067.800723
ATAGAGG19650.067.2611161
ATGTAGG9350.066.905271
GTAGGGT14000.066.804623
TGTAGGG21250.066.792772
Produced by FastQC (version 0.11.5)