FastQCFastQC Report
Mon 27 Feb 2023
SRR3126443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056503
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162080.7881340314115758No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39820.1936296713401342No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG25280.12292712434652418No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23110.11237523115696889No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC22830.111013696551865TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG21770.10585931554682876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57700.082.51871
CGTAGGG5650.073.2277152
ACGGGAT6450.072.133524
ATAGGGA19050.070.3091053
ATAGCGG3900.069.969871
AGGGATG32150.069.872875
TAGGGCA11350.069.562494
AGGGCAT18850.068.5619355
GTACGGG4950.068.3862152
GAATAGG12750.068.2667541
AGGGACT32150.067.8263855
ACGGGTA2150.067.7617954
CTACGAC6050.067.581155
GTAGGGC9150.066.770393
AAGGGTA13500.066.490754
AGAGGGC25950.066.46463
GGTAAGG11150.066.2479551
AGTAGGG31950.065.9247362
GTAGGGA15150.065.7635043
TAGGGAT14650.065.441654
Produced by FastQC (version 0.11.5)