FastQCFastQC Report
Mon 27 Feb 2023
SRR3126442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108423
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166750.7908754552573178No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40220.190758685519936No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG27620.1309983812546154No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC25820.1224611949309982TruSeq Adapter, Index 14 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24180.11468286961392472No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG22470.10657254260648835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59200.082.71751
AGGGATG32200.074.868165
CGTAGGG5750.072.7339552
ATAGCGG4100.072.281161
ACGGGAT6050.071.460834
TAGGGAT12450.070.584264
AGGGTAC9200.070.48995
ATAGGGA18650.070.048943
AGGGAAT23050.069.521495
AAGGGTA13800.069.127784
GGTAAGG12750.068.992171
TAGGGCA10450.068.8035354
CGAAGGG22000.068.564192
GTAGGGC9700.068.30983
AGTAGGG31900.068.203292
AGAGGGC30100.067.757733
TAAGGGA20200.067.232943
TAGCGGG12200.066.6348342
AAGAGGG65600.066.403492
GTAGGGA15400.066.217783
Produced by FastQC (version 0.11.5)