FastQCFastQC Report
Mon 27 Feb 2023
SRR3126441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363039
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211220.8938489800633844No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45490.19250634458424087No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG30480.12898644499731066No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28020.1185761216805986No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC27230.11523296907076015TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG26610.11260922904784898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73900.083.628321
ATAGCGG4100.073.417321
AGGGATG37100.072.460745
TAGGGAT19150.071.172114
ACGGGAT7750.070.952034
GGTAAGG13500.070.7236561
TAGGGCA12100.070.302994
TAGGGTA7950.069.758244
ATAGAGG14200.069.55581
ATAGGGA22200.069.438693
AGTACGG3000.068.981691
GTACGGG6650.068.553672
AGAGGGC35050.068.5193943
TAGAGGG37350.068.326612
TAGCGGG14250.068.270982
AGGGAAT27200.068.078185
AGTAGGG35100.067.886212
AGGGTAT11600.067.661125
GTAGGGC9450.067.6375353
GGATAGG12100.067.634191
Produced by FastQC (version 0.11.5)