FastQCFastQC Report
Mon 27 Feb 2023
SRR3126440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631281
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136340.8357848831685037No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGGTACA32090.19671656814491187No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31210.1913220346463914No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG29870.18310763136455338No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG29530.18102337978557956No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC29480.18071687220043633No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27430.16815006120956474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46900.085.256481
AGGGATG30500.079.509365
TAGGGCA10150.078.2508544
ACGGGAT5750.077.6469044
GTACGGG3350.077.184972
AGGGAAT21800.077.178255
CGTAGGG5100.076.51082
TAGGGAT13850.074.3126454
GTAGGGA15350.074.092693
GGTAAGG9850.074.024711
GTAGGGT5800.072.11583
GAGGGAT22250.071.815354
CGGGAAT4000.071.670145
AGGGTAT9400.071.495155
AGTAGGG26750.071.35382
ATAGGGA14850.071.207293
AGGGTAC8450.070.634265
GAGGGTA9050.070.6250464
GTAGGGC8450.070.078093
AGGGCAT17450.070.02395
Produced by FastQC (version 0.11.5)