FastQCFastQC Report
Mon 27 Feb 2023
SRR3126439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691051
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139550.82522644201742No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG34280.2027141700634694No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC34170.20206368702067531No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG33500.19810165394183854No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31860.18840354312199928No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGGTACA29490.1743885902908901No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27460.1623842214102354No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT17530.10366334309255014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50000.084.3879551
CGTAGGG5450.083.641562
TAGGGCG3000.079.8906254
AGGGATG30550.078.144685
TAGGGTA6650.077.028564
ACGGGTA1100.076.900074
GTAGGGT6500.075.191183
GTACGGG3450.074.918752
TAGGGTC3600.074.4079364
AGGGTAC7650.073.716845
ATAGGGC12600.073.47553
AGGGAAT21200.073.373475
GTAGGGA15750.072.20743
ATAGCGG2850.070.9711151
GAGGGAT24400.070.876934
AGTAGGG27800.070.829822
GGATGGC27950.070.7859657
TAGGGCA11000.070.491734
AGGGTAT9000.069.9695745
ATAGGGA15650.069.966233