FastQCFastQC Report
Mon 27 Feb 2023
SRR3126438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872853
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174350.9309326466092107No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG38830.20733074085366016No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG38120.20353973323053118No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC37960.20268542165348802No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35460.18933680326218874No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31100.16605681278776285No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT20010.10684234160395932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55850.084.805881
CGTAGGG6950.079.798012
TAGGGCA8950.074.569324
AGGGATG33800.074.392985
ACGGGAT5400.073.110614
AGGGTAT10000.071.909515
GTAGGGA16900.071.750993
TAGGGTA5250.071.618564
AGAGGGC30000.071.4395143
AAGGGAC19850.071.0322654
TAGGGAT14500.070.9857254
ATAACGG1400.070.552261
AGGGTAC8800.070.499525
GGTAAGG10550.070.44081
TAGAGGG32600.069.922842
AGGGAAT27700.069.905665
ATAAGGG24350.069.67922
GAGGGTA9800.069.540344
GTACGGG3400.069.117182
AGGGACT27700.069.057295
Produced by FastQC (version 0.11.5)