FastQCFastQC Report
Mon 27 Feb 2023
SRR3126437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606619
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215621.3420730117096835No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22440.1396721935941253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65200.085.896121
ATAGCGG3550.076.890691
ACGGGAT4500.073.105664
ATAGGGC11350.070.80533
GGATAGG8900.070.3292161
CGTAGGG4200.069.418992
AGTAGGG30100.068.117062
AGGGCAT16550.067.300125
TAGACGG2250.066.9332051
CGGGTAT2250.066.839465
TAGGGCA9300.066.704714
TATAGGG13500.066.184422
AAGAGGG57700.066.015382
AGTAAGG11800.065.807611
GAATAGG10800.065.8002241
TAGAGGG32200.065.4271552
ATGAGGG25300.065.055412
ATAAGGG25550.064.602912
GAGGGTA9050.064.3934
CGAAGGG18850.064.114712
Produced by FastQC (version 0.11.5)