FastQCFastQC Report
Mon 27 Feb 2023
SRR3126435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863493
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267351.4346713403270095No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26510.14225972407731072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81100.085.9637151
TAGAGGG37300.071.316062
TAGGGCA11600.071.307374
TATAGGG15950.070.423392
ATAGGGC12300.070.3060153
ATAGCGG3800.068.087421
TAAGGGT9950.068.0172653
GTAGGGC8600.067.2181243
AGAGGGC31750.066.907453
AAGAGGG68200.066.70692
AGTAGGG33400.066.6978152
AGGGCAT18100.066.2127155
AAGGGTA12500.066.173244
GAATAGG13750.066.0301741
TAGGGTA6350.065.871274
TGTAGGG13300.065.3732152
AGGGATG23950.065.149685
CGTAGGG4050.064.984952
AAGGGCA29950.064.651734
AGTAAGG14050.064.285451
Produced by FastQC (version 0.11.5)