FastQCFastQC Report
Mon 27 Feb 2023
SRR3126434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876297
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276561.473967074508993No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21800.11618629673234035No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT19130.1019561402059482No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT18990.10120998967647445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99500.087.2537161
TAGGGTA7500.073.939334
ATAGGGC13900.072.690633
GAATAGG11550.072.111071
CGGGTAT1500.072.059525
TAGGGCA13850.071.9351044
ACGGGAT4550.071.2676544
TATAGGG15850.070.296852
TAAGCGG3950.070.285471
GTAGGGC12900.069.946553
AGTAGGG32050.069.6758652
AAGGGTA14900.068.758284
AGGGATG31250.068.725985
TAGCGGG11500.068.679682
AGAGGGC29550.067.908663
ATAGGGA20450.067.792783
CGTAGGG5700.067.632562
AGGGTAC10250.066.93975
AGGGAAT23400.066.8779755
GTACGGG4450.066.557552
Produced by FastQC (version 0.11.5)