FastQCFastQC Report
Mon 27 Feb 2023
SRR3126433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929746
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276621.4334529000189662No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22360.1158701715148004No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT19970.10348512187614328No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT19400.10053136526776063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102700.087.15271
TAGGGCA12450.074.352034
ACGGGAT6500.072.290734
ATAGGGC14300.071.63543
CGTAGGG5050.070.717912
ATAGGGA18250.070.549663
ATAGCGG3650.069.584651
TAGCGGG10150.068.517722
AGGGATG31550.068.063275
GTAGGGC11050.068.04013
AGGGTAC9350.067.845055
TAGGGTA7850.067.640184
CGAAGGG20750.067.484712
AAGGGTA14300.067.0332264
AGATAGG11250.066.892891
AGAGGGC29750.066.654993
ATGCGGG9800.066.649362
AAGGGAT32700.066.531794
GTAGGGT8550.066.500743
TGTAGGG14050.066.2210542
Produced by FastQC (version 0.11.5)