FastQCFastQC Report
Mon 27 Feb 2023
SRR3126432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166288
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347551.6043573153708093No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25500.11771288028184618No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT22290.10289490594048437No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT21800.10063297216251947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT122800.088.709231
TAGGGCA15850.080.656114
ATAGGGC16250.075.199973
ACGGGAT6000.074.416634
TAAGGGA19600.072.897923
GTAGGGC13000.072.6692053
AGGGCAT23350.072.059925
ATAAGGG30300.071.818442
AGGGATG35700.071.224055
ATAGCGG6150.071.127321
GAATAGG13600.070.207861
ATAGGGA21600.070.064783
AGTAGGG36700.069.923672
TATAGGG17600.069.698832
TAGCGGG12850.069.494132
AGGGAAT25550.068.798415
GGTAAGG11350.068.3780441
CGTAGGG7050.067.999972
TAGGGTA7350.067.782284
AAGAGGG74300.067.3687442
Produced by FastQC (version 0.11.5)