FastQCFastQC Report
Mon 27 Feb 2023
SRR3126431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279955
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212050.9300622161402308No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24890.10916882131445577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78250.086.1901861
CGTAGGG6650.076.378992
TAGGGCA14650.074.747184
ATAGGGC14750.072.9659043
ACGGGAT6050.072.244384
AGGGATG41900.071.225615
ACGGGTA3000.070.496544
GTAGGGC11650.070.193973
ATAGAGG16600.070.030121
AGTACGG3000.069.028341
TAGAGGG45550.068.350462
TAGCGGG14850.068.090082
ATAGGGA23900.068.038493
GTACGGG5050.067.983472
AGAGGGC34150.067.159213
GAGGGAT35450.066.022144
AAGGGCG10400.065.977524
GGTAAGG13750.065.719541
AATAGGG34350.065.71832
AGTAGGG36000.065.7108842
Produced by FastQC (version 0.11.5)