FastQCFastQC Report
Mon 27 Feb 2023
SRR3126430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351674
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212760.9047172354671609No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24220.10299046551520322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78400.084.158561
CGTAGGG7800.077.714282
ACGGGTA2950.074.865474
AGGGATG41050.073.260955
ACGGGAT7150.071.635154
AGTAGGG38550.071.064042
ATAGCGG6150.069.586181
ATAGGGC14000.069.478113
GTAGGGA21700.069.294053
GTAGGGC12300.069.147933
ATAGGGA23550.069.038433
TAGGGCA14100.068.985364
AGGGCAT24300.067.874495
AGGGAAT25950.067.180355
TAAGGGA21000.067.128613
GCATAGG6450.067.078731
TAGGGTA7850.067.04314
AGAGGGC32400.067.0042953
TAGAGGG44350.066.644262
GAATAGG15050.066.557941
Produced by FastQC (version 0.11.5)