FastQCFastQC Report
Mon 27 Feb 2023
SRR3126429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642609
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267311.0115382184802972No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28140.10648567381704975No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26460.10012832015633036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94500.085.652791
CGTAGGG7700.081.78732
ACGGGTA3150.076.089244
TAGGGCA15350.074.091874
GTAGGGC13850.071.257913
AGGGATG46600.070.8978355
AGTAGGG42450.068.862772
ATAGGGC16200.068.463743
TAGCGGG17100.067.884822
TAAGGGA24600.067.246733
TAGAGGG52000.067.151742
GAGGGAT42350.067.026594
ATGAGGG41550.066.847982
GTAGGGA24750.066.6492843
ATAGGGA27550.066.528333
ACGGGAT7850.066.453354
AATAGGG41900.066.289582
TAGGGTA7600.066.165834
TAGACGG3550.064.917251
ATGCGGG13700.064.4924852
Produced by FastQC (version 0.11.5)