Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126428.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2058355 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 15380 | 0.747198612484241 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 3279 | 0.1593019668618873 | No Hit |
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG | 3168 | 0.15390931107607775 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 3029 | 0.1471563457226766 | No Hit |
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC | 2764 | 0.1342819873151133 | TruSeq Adapter, Index 14 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG | 2505 | 0.12169912381489102 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT | 2484 | 0.12067889163919733 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC | 2478 | 0.12038739673185625 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 5885 | 0.0 | 81.31862 | 1 |
ACGGGAT | 585 | 0.0 | 77.92411 | 4 |
ATAGCGG | 510 | 0.0 | 74.73608 | 1 |
TAGCGGG | 1280 | 0.0 | 73.83165 | 2 |
AGGGATG | 3245 | 0.0 | 73.715546 | 5 |
TAGGGCA | 935 | 0.0 | 73.3833 | 4 |
AGGGCAT | 1805 | 0.0 | 70.5583 | 5 |
TAAGGGA | 1850 | 0.0 | 69.85813 | 3 |
ATAGGGA | 1890 | 0.0 | 68.87696 | 3 |
AGGGAAT | 2415 | 0.0 | 68.49857 | 5 |
AGAGGGC | 2855 | 0.0 | 68.47676 | 3 |
AGGGCTC | 1670 | 0.0 | 68.10122 | 5 |
AGTAGGG | 3285 | 0.0 | 67.699 | 2 |
ATAGAGG | 1240 | 0.0 | 67.5482 | 1 |
GAATAGG | 1155 | 0.0 | 67.22293 | 1 |
TGAAGGG | 3515 | 0.0 | 67.01452 | 2 |
GGATGGC | 2700 | 0.0 | 67.01206 | 7 |
ATGAGGG | 3165 | 0.0 | 66.551956 | 2 |
AGGGACT | 2975 | 0.0 | 65.87264 | 5 |
GTAGGGC | 825 | 0.0 | 65.508835 | 3 |