FastQCFastQC Report
Mon 27 Feb 2023
SRR3126428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058355
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153800.747198612484241No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32790.1593019668618873No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG31680.15390931107607775No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30290.1471563457226766No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC27640.1342819873151133TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG25050.12169912381489102No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT24840.12067889163919733No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC24780.12038739673185625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58850.081.318621
ACGGGAT5850.077.924114
ATAGCGG5100.074.736081
TAGCGGG12800.073.831652
AGGGATG32450.073.7155465
TAGGGCA9350.073.38334
AGGGCAT18050.070.55835
TAAGGGA18500.069.858133
ATAGGGA18900.068.876963
AGGGAAT24150.068.498575
AGAGGGC28550.068.476763
AGGGCTC16700.068.101225
AGTAGGG32850.067.6992
ATAGAGG12400.067.54821
GAATAGG11550.067.222931
TGAAGGG35150.067.014522
GGATGGC27000.067.012067
ATGAGGG31650.066.5519562
AGGGACT29750.065.872645
GTAGGGC8250.065.5088353