FastQCFastQC Report
Mon 27 Feb 2023
SRR3126426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318401
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193500.8346269691912659No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36550.15765176084723911No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG36500.15743609496372715No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34330.1480761956193083No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC34150.14729979843866528TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG31230.13470491084156708No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT28820.12430981525629087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68700.084.907671
TAGGGCA12400.076.182184
ACGGGAT7250.075.196734
AGGGATG37350.074.8695765
CGTAGGG7000.071.8397452
TAGCGGG11450.071.420492
TAAGGGA20550.071.125873
GTAGGGC9950.070.85123
GGATGGC30900.070.725097
ATAGGGA22100.070.603273
ATAGCGG4000.070.5623861
AGTAGGG39750.070.349152
ATAGGGC15800.070.1994863
AGGGACT34500.070.020155
AGGGAAT28700.069.268775
TAGAGGG39200.069.178122
AAGGGAC20300.068.992084
TGTAGGG17400.068.876332
TATAGGG17950.068.860522
AGAGGGC31500.068.184343
Produced by FastQC (version 0.11.5)