FastQCFastQC Report
Mon 27 Feb 2023
SRR3126425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256191
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165580.7338917671420548No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32560.14431402305921795No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23780.10539887802052218No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG22580.10008017938197608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66350.083.326811
ACGGGAT8450.075.64144
ATAGGGC16350.075.311443
CGTAGGG6100.073.998162
GGTACGA900.073.1076667
TAGGGCA13700.072.383454
ACGGGTC2950.071.6915364
AGGGCAT22200.069.861575
ATAGGGA25750.069.538473
AGGGATG40850.068.914745
AGTAGGG39000.068.600492
AGTAAGG14150.068.168731
TAGGGAT20400.067.962494
GACCGAT1800.067.885698
GTAGGGC11650.067.773633
CGAAGGG23400.067.7163542
AGAGGGC31500.067.587293
AAGAGGG74100.067.388512
AGGGACT34000.067.1791845
ACGGGTA3150.067.139694
Produced by FastQC (version 0.11.5)