FastQCFastQC Report
Mon 27 Feb 2023
SRR3126424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312780
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166880.7215558764776588No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32560.14078295384775033No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC24750.10701406964778318TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG24260.10489540725879677No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23710.10251731682217936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65450.083.418021
GACCGAT1550.081.8523568
GTAGGGC11050.077.39253
CGTAGGG5750.076.8142
TAGGGCA14300.074.918414
AGGGATG41700.074.708025
ATAGGGC14850.074.0421753
AGTAGGG40050.071.683482
ACGGGTA2500.071.4222264
ATAGGGA25600.070.849553
ACGGGAT7950.070.3346944
AGGGTAC10800.069.6123055
AGTAAGG13200.069.4701161
AGGGCAT22750.069.398115
ATAGCGG4050.068.50691
TAGACGG3450.068.153511
CGAAGGG23250.067.904212
AATGCGG5000.067.717321
GTAGGGT9300.067.7035753
GTACGGG6000.067.348452
Produced by FastQC (version 0.11.5)