FastQCFastQC Report
Mon 27 Feb 2023
SRR3126423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593567
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208940.8056086463160581No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37140.14320046484243515No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28300.10911613233820448No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC27660.10664848835599774TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG26660.1027927946337997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75050.085.5463261
GACCGAT1750.080.567388
ATAGGGC19150.074.36183
TAGGGCA15750.073.704244
CGTAGGG7950.073.30452
ACGGGAT10950.072.964374
AGGGCAT25100.072.8369755
AGGGATG45900.070.957225
GTAGGGC12450.069.8358463
AAGGGCA43000.068.4198154
AGGGATC25200.067.698985
AGTAGGG44150.067.489242
ACGGGTA3000.067.363284
GGTAAGG16850.067.272351
ATAGGGA29400.066.819773
AGTAAGG16800.066.632661
TAGAGGG48550.066.503362
ATAGAGG19250.066.459591
AGGGACT35400.066.2480855
GTAGGGA23100.066.1222153
Produced by FastQC (version 0.11.5)