FastQCFastQC Report
Mon 27 Feb 2023
SRR3126422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254723
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276571.2266251774608234No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34230.15181465749894776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96750.086.0572361
AGTAGGG36150.070.7597962
ATAGGGC14900.069.7045443
TAAGGGA20650.069.639643
CGTAGGG6800.069.1491852
GTAGGGC13450.068.8333743
AGGGATG31100.067.8486255
ATAGAGG16600.067.480671
GTAGGGA17850.067.3995363
AAGAGGG81150.066.57752
TAGGGCA13200.066.576784
TAGAGGG42350.066.285252
ATAGGGA20050.066.0982363
CTAACGG2500.065.892891
AGTAAGG14550.065.666461
CGAAGGG23000.065.625582
GGTAAGG14000.065.55671
AGGGCAT19600.065.457855
AGGGCTT25300.065.384875
AGAGGGC34900.065.3087543
Produced by FastQC (version 0.11.5)