FastQCFastQC Report
Mon 27 Feb 2023
SRR3126421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304201
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281181.2202928477159762No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33870.14699238477893203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99350.087.070961
GTAGGGC12700.072.1549453
TAGGGCA13850.070.5746464
ACGGGTA2600.070.489834
CGTAGGG7900.070.190882
TAAGGGA20400.070.0291063
GGTAAGG14950.069.850781
ATAGGGC16350.069.843143
AGTAGGG38550.069.3607252
GAATAGG12200.068.245331
AAGAGGG80000.067.198842
ATAACGG2100.067.1987841
GAGGGAT25350.067.106694
ATAGCGG5900.066.970991
ATAGGGA21150.066.8792343
AGAGGGC33750.066.556323
ATAAGGG31900.066.437242
AGATAGG13900.066.328591
ATGCGGG12650.066.1234052
TAAGAGG16300.066.085681
Produced by FastQC (version 0.11.5)