FastQCFastQC Report
Mon 27 Feb 2023
SRR3126420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592416
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347571.3407184649377262No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39870.15379476133460063No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC25950.10009967536074456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112350.088.303071
ATAGCGG8250.072.4139251
ATAGGGC18550.071.448323
AGTAGGG41850.070.9754942
TAGGGCA16100.070.352244
CGTAGGG7900.070.2007452
TAAGGGA26050.069.460813
ATAGGGA23550.069.0513153
AGGGATG38800.068.923515
TAGACGG3550.068.904451
GTACGGG7100.068.8433152
AGATAGG15900.068.341921
ATAGAGG19450.068.2027361
AGGGTAA11200.067.560785
TAGAGGG48950.067.1137162
ATAAGGG38000.066.540412
GAGGGAT31950.066.48974
GAATAGG13750.066.369931
TAGCGGG17450.065.9868552
AAGGGTA19100.065.945974
Produced by FastQC (version 0.11.5)