FastQCFastQC Report
Mon 27 Feb 2023
SRR3126419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166611
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193720.894115279577183No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49380.22791354793269303No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG25090.11580297524567171No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC25050.11561835511773919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67100.083.877291
CGTAGGG6850.078.933582
ACGGGAT6200.075.042554
ATAGAGG14150.074.4950261
TAGGGCG4450.073.9268344
ATAGCGG3800.071.825741
ATAGGGC14800.071.12963
ATAGGGA19800.069.782453
AGGGTAC9000.068.4057855
AGGGATG29200.068.2412645
AGTAGGG35750.067.862242
AAGGGTA16450.066.8519444
ATAAGGG31450.066.526382
AGAGGGC31300.066.2152943
TAGGGTC6050.066.027924
AGGGACT35100.065.7413255
TAGTAGG8450.065.714481
TATAGGG18400.065.4148942
AGTAAGG12700.065.214581
AAGGGAC19750.064.9620364
Produced by FastQC (version 0.11.5)