FastQCFastQC Report
Mon 27 Feb 2023
SRR3126418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242054
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194770.8687123503715789No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49770.22198394864708879No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC28320.12631274715060387No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG26350.11752616127889873No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG23290.103877961904575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66350.082.6647641
ATAGAGG15300.072.865121
ATAGGGC15050.071.821893
CGTAGGG6300.070.117072
TATAGGG20200.070.024752
ATAGCGG4450.069.7664641
TAAGAGG14750.068.884991
TAGGGCA11400.068.845754
TGTAGGG16200.068.459462
AGTAGGG37400.067.851372
ATAACGG2100.067.19931
AGGGATG27800.066.7756045
ACGGGAT6200.066.7047044
AGATAGG12100.066.4773251
ATAGGGA19500.066.2771153
ATGAGGG35400.066.242042
TAGGGAT14550.066.2150044
AGGGAAT20750.066.134835
AGGGTAC10550.065.9287345
GTAGGGA16400.065.623163
Produced by FastQC (version 0.11.5)