FastQCFastQC Report
Mon 27 Feb 2023
SRR3126417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492013
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242990.9750751701536067No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56260.2257612620800935No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC33230.1333460138450321No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG27630.1108742209611266No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG25850.10373140108017093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79650.083.665071
ACGGGAT6900.074.243524
ATAGGGC16100.071.8109053
TAGGGCA12750.070.4051364
GAGGGAT25800.069.404314
ATAGAGG16000.069.366871
CGTAGGG8750.068.751642
AGTAGGG41300.068.619642
TAGGGTC7250.068.0663454
TAGAGGG45450.066.4902952
ATAGGGA19450.066.449893
AATAGGG36750.066.245072
TATAGGG20500.065.5682142
GGTAAGG15750.065.391851
GTAGGGT10350.065.388793
AAGGGTA17800.064.688524
TAGGGAT15050.064.642044
AGAGGGC36750.064.582553
AGGGATG32750.064.5776755
AAGAGGG81450.064.568422
Produced by FastQC (version 0.11.5)