FastQCFastQC Report
Mon 27 Feb 2023
SRR3126416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567042
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307311.1971366265140968No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40730.1586651094917808No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT32860.12800725504296384No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG28880.11250302877786962TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC25850.10069956003836322TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102100.086.0686341
TAGGGCA13100.074.620484
ATAGGGC15150.073.829563
ATAGGGA19950.070.671513
GTAGGGT8450.070.07773
AGTAGGG40700.068.508792
TAAGGGA21150.068.217223
GTAGGGA19800.068.121273
GTAGGGC12100.067.581823
GAGGGAT29650.067.2058644
ATAGCGG5550.066.997781
ACGGGAT7250.066.767524
AGAGGGC34350.066.766573
CCGTACA8250.066.649663
AAGAGGG82500.066.5694352
TATAGGG21900.066.34382
GATAGGG34800.066.206832
AGATAGG13450.066.140251
AGGGATG36300.066.028225
CGTAGGG6200.065.9801942
Produced by FastQC (version 0.11.5)