FastQCFastQC Report
Mon 27 Feb 2023
SRR3126415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646115
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313431.1844912258159603No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40280.15222316490401966No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT34150.12905712714677933No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30670.11590577129111926TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC28380.10725157447805556TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99000.086.132921
ACGGGAT7050.074.650574
TAGGGCA14350.072.695134
AGTAAGG15600.070.550131
ATAGGGC15450.069.956533
CGTAGGG7600.069.8704762
TAAGGGA22550.068.144393
GAATAGG15750.068.086481
AGATAGG14500.067.468631
GTAGGGA20350.067.1981663
ATAGGGA21450.067.03833
AGTAGGG41500.067.035142
GTAGGGC13850.066.841373
GATAGGG36700.066.327342
AGGGAAT24950.065.9176565
GGTAAGG15150.065.815741
GAGGGAT28900.065.525674
AGGGACT36950.065.4933245
ATAGAGG18200.065.123191
TATAGGG19150.065.028842
Produced by FastQC (version 0.11.5)