FastQCFastQC Report
Mon 27 Feb 2023
SRR3126414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947783
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389151.3201446646513668No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47940.16263069567875246No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT36330.12324516424716474No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG32130.10899716838044049TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123450.086.325471
CGTAGGG8200.073.362052
ATAGGGA22950.072.289423
GTAGGGA21450.071.647863
TAGGGCA16400.071.6438454
ACGGGAT6750.071.0197454
TAGGGTA8100.070.787664
GTAGGGT10300.070.26943
ATAGGGC18300.069.855493
GTAGGGC14250.069.590553
TATAGGG25450.069.434522
AGTAGGG47050.068.723332
ATAGCGG5550.068.637611
GAATAGG17550.068.333431
TAAGGGA25100.068.156993
ACGGGTA2850.067.611694
AGTAAGG16700.067.587271
ATAGAGG21400.067.467521
AGGGTAG12500.066.9256745
TAGACGG4150.066.861181
Produced by FastQC (version 0.11.5)