FastQCFastQC Report
Mon 27 Feb 2023
SRR3126413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821339
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163620.8983500600382466No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40130.22033240379742597No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG32730.17970295480413037No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG28680.15746656717942129No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC27480.1508780078832112Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAAACAGT23670.12995933211774413No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22930.12589638721841456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54850.082.903661
CGTAGGG5300.077.186112
TACGACC8800.074.23176
ATAGCGG3900.073.627231
CTACGAC8950.073.512685
TAGGGCA8700.073.46444
AGGGAAT23950.072.406595
ATAGGGC12450.071.720213
AGGGATG32750.070.601035
ATAGGGA15950.070.1251143
ACGGGAT6300.070.120424
ATAAGGG24250.069.8052
AGGGCAT15250.069.645955
TAGGGTA5350.069.3953864
TCCGCTA9300.069.344311
AGTAGGG26800.069.128522
TAGGGAT14350.068.7740554
GGATGGC28700.068.6103067
TAAACGG1100.068.470031
GAATAGG10600.068.389281
Produced by FastQC (version 0.11.5)