FastQCFastQC Report
Mon 27 Feb 2023
SRR3126412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858032
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165530.8908888544438417No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40750.2193180741774092No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG37790.20338723983225263No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG32620.17556210011452977No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC31640.17028770225701173Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23120.12443273312838529No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAAACAGT22190.11942743720237327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54000.084.565731
TAGGGTA5750.079.276674
CGTAGGG6500.076.6362152
TACGACC6750.076.5826646
AGGGCAT16800.072.448945
TAGGGCA8850.072.216624
AGGGATG30950.071.819445
AGGGAAT24750.071.772515
CTACGAC7250.071.3010945
GAGGGAT27300.071.265484
AGGGCGA11750.071.1907655
TATAGGG14200.071.152742
ATAAGGG24250.070.926892
AGTACGG1750.069.872161
CGGGAAT3850.069.575395
GTAGGGA15150.069.4846043
TAGGGCG3200.069.02234
TAAGGGA15350.068.885433
AGTAGGG27250.068.8094252
ATAGGGC11950.068.4281163
Produced by FastQC (version 0.11.5)