FastQCFastQC Report
Mon 27 Feb 2023
SRR3126411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060513
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207181.005477762091285No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46450.22542929843199244No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG40990.19893104290048158No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG36110.17524762037414954No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC35460.17209306614420777Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26180.12705573806134685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62850.083.895581
CGTAGGG6700.076.4632952
ACGGGAT5650.075.699724
AGGGATG35250.075.467275
TACGACC8350.074.861056
TAGGGCA10050.072.020474
CTACGAC8750.071.4405755
TAGGGTA6300.070.873594
GAGGGAT29450.070.859664
ATAGGGA18750.070.688573
TAGGGAT15050.070.5786364
AGGGAAT25450.070.1773765
GTAGGGA14800.069.8654253
TAAGCGG4250.069.7252961
AAGGGTA11500.069.478824
ATAGCGG4000.069.379431
AGTAAGG13050.069.203721
TAGAGGG32000.069.178682
AGGGTAC9450.069.132835
AAGGGAT37650.069.033754
Produced by FastQC (version 0.11.5)