FastQCFastQC Report
Mon 27 Feb 2023
SRR3126410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467446
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48411.0356276446905097No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA46280.9900608840379423No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG22920.49032401603607684No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC20560.4398369009468473No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG19390.41480727185600047No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17530.37501657945516703No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG15330.3279523196262242No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13680.2926541247545171No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAACGGAATC10850.2321123723381952No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT10110.216281666759369No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT9740.20836631396995586No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAATCG7720.16515276630883566No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG7510.16066026877970932No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7120.15231705908276036No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT6690.1431181355707397No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6610.14140670794059634No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT6530.13969528031045297No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT6140.13135207061350404No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC6050.12942671452959273No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5940.1270735015381456No Hit
AAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5500.11766064957235703No Hit
TAGACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT5340.11423779431207026No Hit
GATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC5290.11316815204323066No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5120.10953136832917598No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC5120.10953136832917598No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT5090.10888958296787223No Hit
GGAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5040.10781994069903261No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4720.1009742301784591No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG156.849502E-494.172581
CTAACGG156.849502E-494.172581
GGTTATC156.905124E-493.981187
GGTATGA600.086.1494147
ATAACGG550.085.611441
CGTTTTT26950.085.2621
CGGGTAT500.084.583055
TAGGGTA1100.081.165564
AGGGCAA3900.080.7274255
GTACGGG700.080.624292
ACGGGTA353.8649887E-880.555294
CGCTACG3500.080.555293
CGTAGGG950.079.209822
GTAGGGA3800.079.142043
AGGGTAC1250.078.944185
AGGGAAT8750.078.407155
CGTACCC301.4565176E-678.317654
TGTGAGG1150.077.794741
AGTGCGG1150.077.794741
TAGGGCA2000.077.534474
Produced by FastQC (version 0.11.5)