FastQCFastQC Report
Mon 27 Feb 2023
SRR3126409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486631
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49461.0163758576827207No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA43480.8934901393458287No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG25530.5246274898228842No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC21750.4469505641851834No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG20650.424346167835588No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18680.38386374891858516No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG13990.28748682266440073No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12500.2568681403363123No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAACGGAATC10260.21083736958804516No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT10170.2089879189776237No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT9960.20467253421997367No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAATCG8520.17508132445323046No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT7780.15987473054532078No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG7520.1545318732263255No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT7050.14487363114968013No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6580.1352153890730348No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT6450.13254396041353717No Hit
GATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC6350.13048901529084667No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6320.1298725317540395No Hit
TAGACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT6190.12720110309454186No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC5910.12144725675100845No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT5700.11713187199335841No Hit
AAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5600.11507692687066791No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5450.11199450918663216No Hit
AATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5450.11199450918663216No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5440.11178901467436313No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC5250.10788461894125118No Hit
GGAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5160.10603516833082972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG201.8343546E-594.02551
CTAGCGG409.094947E-1294.02551
GACCGAT254.9240225E-793.977198
CGTTTTT26250.087.9362261
CGTAGGG1300.086.748182
AGGGTAT2900.085.875715
AGTACGG452.7284841E-1183.578221
GCGTAGG401.031367E-982.272311
GGTATGA401.036824E-982.230047
GGGTATC2500.080.820386
CGGGTAT700.080.551885
AAGGGTC1350.080.054644
AGGGATG7400.079.372625
TAGGGCA2200.079.0262764
GTACAGG900.078.3545761
TAAGGGT1500.078.314323
TCCGCTA3100.077.343551
AGGGAAT10000.077.0612955
TAGGGTA1650.076.890434
CGCTACG3000.076.748033
Produced by FastQC (version 0.11.5)