FastQCFastQC Report
Mon 27 Feb 2023
SRR3126408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536923
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63751.1873210870087518No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG29320.5460745768015153No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG24600.4581662547516125No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC23620.4399141031395563No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21800.4060172501457378No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA18970.3533095062048003No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT12580.23429802783639367No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT12130.22591693781044953No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT9070.16892552563402946No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAATCG8900.1657593360686728No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG8300.15458454936741395No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT7940.14787967734665863No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC7480.13931234087569355No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7180.13372494752506411No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA7120.1326074688549382No Hit
TAGACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT6870.1279513077294137No Hit
AAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT6740.12553010394414096No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG6610.12310890015886822No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC6550.12199142148874233No Hit
GATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC6490.12087394281861646No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT6200.11547279591300802No Hit
AATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT6180.11510030302296605No Hit
GGAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5720.10653296655200094No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG5530.1029942840966023No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG5510.10262179120656034No Hit
GACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC5490.10224929831651838No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5460.10169055898145545No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG156.878287E-494.075221
GTCGAGT156.891039E-494.031494
TACGGGT353.583409E-1093.996373
GACGTCA156.9038087E-493.987628
CGTAGGG1150.089.909572
CGTTTTT32150.088.515571
ACGGGTA850.082.937974
CGAGGGA2800.082.2468263
TAGGGCA2500.080.8368764
AGGGCGC700.080.568325
AGGGTAT3100.080.351735
GTTACGG650.079.602111
CGCTACG4850.079.4608463
AAGGGTA2900.079.410734
AGGGAGT2550.079.251845
AGGGTCC2200.079.04245
GTAGGGT1800.078.330313
TAACGGG1500.078.330312
TATCGGG301.4554153E-678.330312
CGAAGGG5050.078.175192
Produced by FastQC (version 0.11.5)