FastQCFastQC Report
Mon 27 Feb 2023
SRR3126407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797897
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144940.8061640906014081No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25410.14133178930717388No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC24360.1354916327242328No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG24200.13460170410207037No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG21790.12119715423074848No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20200.11235348854800914No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC18160.10100689861543793TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52350.083.506931
CGTAGGG5150.082.173612
CGAAGGG16350.077.075112
ATAGCGG2700.076.685351
ACGGGTA1850.073.668484
AGGGAAT23450.073.5493245
AGGGATG30450.073.4640965
AGGGTAC8400.072.7309955
TAAGGGA17150.072.342793
TAGGGAT13050.070.5831454
AAGGGAT33700.070.562844
GAATAGG10750.070.038211
AGTAGGG29950.070.02212
AGAGGGC24250.069.766393
TAGGGCA10150.068.525324
ATAGGGC11800.068.1035163
AGGGCTC15750.068.031455
AATCGGG1800.067.920042
GAGGGAT24750.067.7873154
TAGCGGG8750.067.711052
Produced by FastQC (version 0.11.5)