FastQCFastQC Report
Mon 27 Feb 2023
SRR3126406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862899
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145780.7825437664629162No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG26780.14375443864643225No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26020.13967477571247824No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG24530.1316764891709105No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC23460.12593275319810682No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20090.10784266887254756No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC19900.10682275313905908TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48250.083.335961
ATAGCGG3600.082.300351
AGGGATG29200.072.091765
TAGGGCA10000.071.422344
CGAAGGG16300.070.624812
TAGGGAT12400.070.103634
AGGGTAC7800.069.880155
TAGGGAC8150.069.761884
TAGCGGG8900.069.688752
GTAGGGA13450.069.5218353
AGGGCTC16100.069.461085
ATAGGGC13300.069.246033
AGTAGGG27700.069.208462
GGGCGAT6900.068.780096
AAGGGAT32900.068.6972964
AGGGAAT23700.068.599075
TAGGGTA6400.068.279984
TAGGGCG4200.068.2450264
TAGAGGG28750.067.661452
ATAGGGA15700.067.340033
Produced by FastQC (version 0.11.5)