FastQCFastQC Report
Mon 27 Feb 2023
SRR3126405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082159
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184930.8881646406446385No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG29450.14143972674517172No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29370.14105551016997261No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG27680.13293893501889145No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23650.11358402504323638No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC22280.10700431619295164TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62400.083.919351
TAGGGCA10600.075.3727954
AGGGTAC8600.074.867555
AGGGATG32150.073.090455
ATAGCGG3800.071.811831
AAGGGAT37450.071.280074
CGAAGGG19150.071.170632
AGGGAAT26750.070.8031855
TAACGGG5850.070.696572
GAATAGG12750.069.743411
GTAGGGT9300.069.737723
TAAGGGA17950.069.6448063
TAGGGAC10550.069.493424
AGTAGGG33200.069.221722
AGGGCAT19100.068.650295
TAGGGCG5700.068.4344564
ACGGGTA1650.068.3595054
AAGGGTA12600.068.2577744
AGGGATT24900.067.192725
TAGGGTG9600.067.0688554
Produced by FastQC (version 0.11.5)