FastQCFastQC Report
Mon 27 Feb 2023
SRR3126404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155998
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329201.5269030861809705No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30900.1433210977004617No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT24860.11530622941208667Illumina PCR Primer Index 2 (95% over 22bp)
AAGAGAGGTGCATGTTGGTATTTAAAGTAGTGGATTCAAAGAACTTAGAT22700.10528766724273399No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT22420.10398896473929939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115200.087.8745651
ACGGGTA2400.076.370194
ATAGGGC13200.070.139533
TAGGGCA11150.068.28274
TAGACGG2900.068.159531
AGTAGGG33100.067.4710542
CCGTACA5650.067.376293
GTAGGGA13500.067.188363
GTACGGG4900.067.166672
GGTAAGG11950.066.557051
ACGGGAT4750.066.2905654
AGGGTAC9650.066.234185
ATAGCGG4550.066.1978451
AGGGCAT18150.065.511035
CGTAGGG5700.065.163462
GTAGGGC9550.064.959273
TAGGGTA6700.064.533254
AGAGGGC33850.064.421363
AAGAGGG72900.064.365762
CGAAGGG20900.063.888682
Produced by FastQC (version 0.11.5)