FastQCFastQC Report
Mon 27 Feb 2023
SRR3126403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184078
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328781.505349167932647No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29470.13493107846880925No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT24620.1127249118392292Illumina PCR Primer Index 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT24120.11043561631040649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118850.087.898231
TAGGGCA11400.075.8483054
ATAGGGC14350.073.6823653
ATAGCGG4300.071.1011
GGTAAGG13150.068.67621
AGTAGGG35900.068.198712
AGTAAGG13350.067.647351
ATAGGGA17450.067.5944753
AAGGGTA12100.066.411554
CGTAGGG5150.065.6989062
GTACGGG5150.065.6989062
GAATAGG12100.065.3062441
AGAGGGC32400.064.977933
CGAAGGG21700.064.967242
GTAAGGG33600.064.475482
GGATAGG12700.064.443091
AAGGGGC65300.064.408434
TAGGGTC5400.064.397774
ACGGGTA2200.064.081334
GTAGGGA14450.064.066543
Produced by FastQC (version 0.11.5)