FastQCFastQC Report
Mon 27 Feb 2023
SRR3126402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440076
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414241.6976520403462843No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34940.1431922612246504No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT26870.11011952086738282Illumina PCR Primer Index 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT26520.1086851393153328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135900.087.842151
CGTAGGG5750.073.56282
ATAGGGC14250.071.899393
AGTAGGG39350.069.75122
TAGAGGG41400.067.546082
TAGGGCA12550.067.4081344
GTAGGGC12500.066.549813
AAGGGAC23600.065.718184
AGAGGGC34150.065.37123
GTAGGGA16700.065.0098343
ATAGGGA21150.064.886753
AAGAGGG82000.064.766162
ACGGGTA2550.064.5076754
ATAGCGG5350.064.180021
AGATAGG14150.064.1551741
TATAGGG22300.064.069642
TAAGAGG17300.063.892891
GTATAGG10400.063.770021
TAGGGTA7750.063.6753274
AGGGACT35900.063.493735
Produced by FastQC (version 0.11.5)