FastQCFastQC Report
Mon 27 Feb 2023
SRR3126401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452873
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235600.9605063123936707No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40970.16702862316964637No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC25230.10285897394606243TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85450.087.176151
ACGGGTA2600.072.300234
TAGGGAT14800.070.810254
GAATAGG15300.068.894641
TAGGGCA14400.068.860944
TAGGGAC12700.068.827544
ATAGGGA21200.068.497643
TATAGGG21100.067.968792
ATAGCGG6050.067.6694641
AATAGGG34200.067.094332
AGGGATG29800.067.0232855
AGTAGGG40350.066.8898852
GTAGGGA17300.066.825473
CGTAGGG6500.066.552842
AGACGGG16400.066.5175552
AAGGGAC24400.066.448064
AAGAGGG82850.066.4026642
ATAGGGC16000.066.3806463
TAGACGG3050.066.343361
GAGGGAT27700.065.487824
Produced by FastQC (version 0.11.5)