FastQCFastQC Report
Mon 27 Feb 2023
SRR3126400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537711
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242220.9544822085730015No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42800.16865592654167477No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC28970.11415799513813828TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG25600.10087831120249706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87200.085.323621
TAGACGG3100.074.338481
ACGGGAT5700.073.3756564
ATAGGGC16550.072.974643
TATAGGG20850.071.6719742
TAGGGCA13600.070.835644
CGTAGGG5400.069.618482
GAATAGG17100.069.308051
AATAGGG37300.069.039892
TAGGGAC14250.068.26414
AGTAGGG38700.068.24232
AGGGATG28250.068.036465
ATAAGGG34300.067.680122
AAGGGAC25300.067.4253164
ATAGGGA22350.067.2836153
GTAGGGA18600.066.952963
TAGGGAT15350.066.7398154
AGGGACT40400.066.651535
ATAGCGG5750.066.251611
AAGAGGG86150.065.7840042
Produced by FastQC (version 0.11.5)