FastQCFastQC Report
Mon 27 Feb 2023
SRR3126399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832753
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303281.0706192880212289No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47890.1690581565000549No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC30810.1087634537850635TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG28520.10067944504868587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109450.086.103581
CGTAGGG7550.073.454982
GAATAGG17150.072.938491
TATAGGG25300.070.776762
ATAGGGC18350.070.69023
ATAGGGA26300.070.408763
TAGAGGG49200.068.874162
AAGGGAC28700.068.1236044
AGGGATG32200.068.016815
AATAGGG41400.067.887062
ATAAGGG39200.067.7405242
AGTAAGG16950.067.6954351
AAGAGGG94100.067.026892
AGTAGGG42200.066.600112
AGGGACT46000.066.411265
GAGGGAT31750.066.16834
ATAGAGG17700.066.15541
ACGGGTA2700.066.146374
ATAGCGG6550.066.051971
GCATAGG9150.065.785191
Produced by FastQC (version 0.11.5)