FastQCFastQC Report
Mon 27 Feb 2023
SRR3126398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031187
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184230.9070065926967827No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36380.17910709353693186No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24160.11894522759352043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70650.084.671721
CGTAGGG6050.081.6018752
TAGGGCA13550.076.303854
ACGGGTA1800.075.716154
AGGGATG46450.073.858455
AGTAGGG36900.073.39432
ATAGAGG12600.073.213391
GGATGGC38700.070.797947
TAAGGGA21450.070.5491263
GTAGGGA19600.070.254573
AAGGGAT42600.070.163394
GAATAGG12800.069.4955141
ACGGGAT5550.068.58914
TAGGGCG3700.068.5890964
ATAGGGC14400.068.536173
GTAGGGC11550.068.358163
TAGAGGG38150.068.278112
GGTAAGG13000.067.340231
TAGGGAC12500.067.2986154
ATAGGGA19800.067.171383
Produced by FastQC (version 0.11.5)