FastQCFastQC Report
Mon 27 Feb 2023
SRR3126397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070766
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186840.9022748103841768No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37550.1813338638938441No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23820.11502989714917088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64250.085.207351
CGTAGGG7350.076.722712
AGGGATG44950.075.279165
TAGGGCA13500.075.195524
ATAGGGC13800.072.8797153
TAGACGG3100.072.824371
GTACGGG3950.072.5709462
ACGGGTA1850.071.13094
ATAGAGG14300.071.0419541
AGTAGGG35750.070.7190252
TAAGGGA18900.070.6201253
GTAGGGA19650.069.359733
GGATGGC34700.069.073697
TAGGGCG3750.068.929234
GTAGGGC12550.068.904273
ATAGGGA19300.068.669493
AGACGGG13000.068.32012
TAGGGAT18350.067.358384
AGGGAAT24900.066.81535
AAGAGGG69550.066.41812
Produced by FastQC (version 0.11.5)