FastQCFastQC Report
Mon 27 Feb 2023
SRR3126396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315053
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232011.0021800796785214No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42560.18384028357018176No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27760.11991086165197946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78050.084.784061
AGGGATG48650.076.419685
TAGGGCA15200.076.0681844
ATAGGGC14650.073.790723
TAGGGCG3950.071.394654
ATAGAGG16300.070.980741
CGTAGGG7200.070.502222
TAGAGGG41800.070.389782
TACGCGG1550.069.789361
ATAGGGA22850.069.7308653
AGTAGGG41150.069.445682
AGGGAAT28750.069.316965
GTAGGGA21600.069.196623
TAGGGAT20250.068.239194
CGAAGGG23900.068.043982
TATAGGG21350.068.025562
TAAGGGA22600.068.006573
AGGGTAC11900.067.9349065
TAGGGTA7200.067.891034
ATGCGGG13450.067.793962
Produced by FastQC (version 0.11.5)